Leishmaniosis 2017-11-15T13:52:06+00:00

OIE Reference Laboratory for Leishmaniosis. National Reference Centre for Leishmaniosis (C.Re.Na.L.)

Protozoan identification

National Reference Centre for Animal Leishmaniasis performs the serological (IFAT, Elisa) and molecular diagnostic (including Real Time PCR) tests applied to Leishmaniasis such as the isolation, characterisation of strains and production of reference materials. Standard Sera (positive and negative) are available such as DNA standards and Leishmania strains.

Details of available exams are reported in the tables below.

The Standard Operative Procedures (POS/PdP) are certified according to UNI CEI EN ISO/IEC 17025:2005.

Molecular diagnostic

Pathogen Matrix Method POS/PdP
Leishmania Infantum Needle aspiration lymph node, bone marrow, blood, spleen (fresh, frozen or refrigerated) Immunofluorescence assay test POS CRE07 Rev.1
Leishmania Infantum Needle aspiration lymph node, bone marrow, blood, spleen (fresh, frozen or refrigerated) Leishmania Isolation
Leishmania spp. Extracted DNA Giemsa staining
Leishmania spp. Extracted DNA Cytotoxis activity evaluation

Serological and antigen identification

Pathogen Matrix Method POS/PdP
Leishmania Infantum Serum (refrigerated) Immunofluorescence assay test PDP CRE02Rev. 5
Leishmania spp. Needle aspiration lymph node, bone marrow (refrigerated max 72h) Leishmania Isolation PDP CRE04

Rev. 5

Leishmania spp. Smear slide (fresh) Giemsa staining
Leishmania spp. Leishmania protozoan Cytotoxis activity evaluation

Services

Immunofluorescence assay (Leishmania Infantum antibody test kit )
Proficiency test organization
Technical training
Research

 

Browse Catalog Contact: Fabrizio Vitale fabrizio.vitale@izssicilia.it

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