{"id":931,"date":"2017-08-08T23:52:46","date_gmt":"2017-08-08T23:52:46","guid":{"rendered":"http:\/\/bbmed.it\/babeiosis\/"},"modified":"2017-12-19T16:24:55","modified_gmt":"2017-12-19T15:24:55","slug":"babeiosis","status":"publish","type":"page","link":"https:\/\/bbmed.it\/it\/babeiosis\/","title":{"rendered":"Babeiosis"},"content":{"rendered":"

Laboratorio di Referenza OIE Reference for Babesiosis e Theileriosis. Centro nazionale di referenza per Anaplasma, Babesia, Rickettsia e Theileria (C.R.A.Ba.R.T.)<\/h3>\n

Agente patogeno la rivelazione del DNA viene effettuata su DNA estratto da sangue, tessuto, colture cellulari e campioni di artropodi. Estrazione del DNA pu\u00f2 essere effettuata a partire da freschi o congelati (- 20\u00b0C ) il sangue e tessuti (milza \u00e8 il pi\u00f9 idoneo organo per il rilevamento del patogeno). Artropodi (zecche o pulci) freschi o conservati in 70% di alcol o in acido nucleid reagente conservazione “RNA seguito ” a temperatura ambiente pu\u00f2 essere analizzata. Pcr vengono eseguite per rivelare il DNA di agenti patogeni appartenenti a Anaplasma, Babesia, rickettsie e Theileria generi. Real Time PCR sono effettuati per A. marginale, B. bovis, B. bigemina e Rickettsias spp. Linea retromarcia blotting viene effettuata per Babesia e Theileria specie nei ruminanti. Il sequenziamento \u00e8 effettuata per confermare la diagnosi e per la specie\/ceppo la digitazione. Dettagli degli esami sono riportati nelle tabelle seguenti.<\/p>\n

La procedura operativa standard (POS\/PdP) \u00e8 certificata UNI CEI EN ISO\/IEC 17025:2005.<\/p>\n

Molecular diagnostic<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Pathogen<\/strong><\/td>\nMatrix<\/strong><\/td>\nMethod<\/strong><\/td>\nPOS\/PdP<\/strong><\/td>\n<\/tr>\n
A. marginale<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nReal-time PCR<\/td>\n–<\/td>\n<\/tr>\n
Anaplasma spp<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 22<\/td>\n<\/tr>\n
A. marginale<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 03<\/td>\n<\/tr>\n
A. ovis<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\n–<\/td>\n<\/tr>\n
A .phagocytopylum<\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 11<\/td>\n<\/tr>\n
All the pathogenes<\/td>\nPurified PCR products<\/td>\nSequencing<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Pathogen<\/strong><\/td>\nMatrix<\/strong><\/td>\nMethod<\/strong><\/td>\nPOS\/PdP<\/strong><\/td>\n<\/tr>\n
B. bovis, B. bigemina<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nReal-time PCR<\/td>\n–<\/td>\n<\/tr>\n
B. bigemina<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 02<\/td>\n<\/tr>\n
B. bovis<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 09<\/td>\n<\/tr>\n
B. ovis, B. canis<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\n–<\/td>\n<\/tr>\n
B. caballi<\/em><\/td>\nBlood, tissue, and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 15<\/td>\n<\/tr>\n
B. microti<\/em><\/td>\nBlood, tissue, and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 21<\/td>\n<\/tr>\n
B. bigemina, B. bovis, B. divergens, B. major, B. motasi, B. ovis, B. crassa<\/em><\/td>\nBlood, tissue, and arthropod samples<\/td>\nReverse line blotting<\/td>\n <\/p>\n

–<\/td>\n<\/tr>\n

All the pathogenes<\/td>\nPurified PCR products<\/td>\nSequencing<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n
Pathogen<\/strong><\/td>\nMatrix<\/strong><\/td>\nMethod<\/strong><\/td>\nPOS\/PdP<\/strong><\/td>\n<\/tr>\n
Rickettsia spp., Rickettsia conorii<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nReal-time PCR<\/td>\n–<\/td>\n<\/tr>\n
Rickettsia spp<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 04<\/td>\n<\/tr>\n
All the pathogenes<\/td>\nPurified PCR products<\/td>\nSequencing<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n
Pathogen<\/strong><\/td>\nMatrix<\/strong><\/td>\nMethod<\/strong><\/td>\nPOS\/PdP<\/strong><\/td>\n<\/tr>\n
T.annulata<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 01<\/td>\n<\/tr>\n
T.ovis<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\n–<\/td>\n<\/tr>\n
T.equi<\/em><\/td>\nBlood, tissue and arthropod samples<\/td>\nPCR<\/td>\nPOS-CRA 14<\/td>\n<\/tr>\n
T. annulata, T. velifera, T. taurotragi,<\/em><\/p>\n

\u00a0T. mutans, <\/em><\/p>\n

T. buffeli\/orientalis,<\/em><\/p>\n

T. ovis, T. lestoquardi, T. hirci<\/em><\/td>\n

 <\/p>\n

Blood, tissue, and arthropod samples<\/td>\n

Reverse line blotting<\/td>\n <\/p>\n

 <\/p>\n

–<\/td>\n<\/tr>\n

All the pathogenes<\/td>\nPurified PCR products<\/td>\nSequencing<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Serological diagnostic<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Pathogen<\/strong><\/td>\nMatrix<\/strong><\/td>\nMethod<\/strong><\/td>\nPOS\/PdP<\/strong><\/td>\n<\/tr>\n
A. marginale<\/em><\/td>\nRuminants serum<\/td>\nELISA assay<\/td>\nPOS-CRA 07<\/td>\n<\/tr>\n
B. bigemina<\/em><\/td>\nSerum of animals receptive<\/td>\nELISA assay<\/td>\nPOS-CRA 18<\/td>\n<\/tr>\n
A. phagocitophilum<\/em><\/td>\nSerum of animals receptive<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 12<\/td>\n<\/tr>\n
B. canis<\/em><\/td>\nDog serum<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 16<\/td>\n<\/tr>\n
B. bovis<\/em><\/td>\nSerum of animals receptive<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 20<\/td>\n<\/tr>\n
B. microti<\/em><\/td>\nSerum of animals receptive<\/td>\nImmunofluorescence assay<\/td>\n–<\/td>\n<\/tr>\n
B. caballi<\/em><\/td>\nHorse serum<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 10<\/td>\n<\/tr>\n
R. conorii<\/em><\/td>\nSerum of animals receptive<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 05<\/td>\n<\/tr>\n
T. equi<\/em><\/td>\nHorse serum<\/td>\nImmunofluorescence assay<\/td>\nPOS-CRA 13<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Plasmid DNA testing <\/strong><\/p>\n\n\n\n\n
\u00a0<\/strong><\/td>\nMethod<\/strong><\/td>\n<\/tr>\n
PCR products starting from any pathogen<\/td>\nCloning<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Services<\/strong><\/p>\n\n\n\n\n\n
Proficiency test organization<\/td>\n<\/tr>\n
Technical training<\/td>\n<\/tr>\n
Research<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
Visualizza Catalogo<\/a><\/td>\nContatta: Dr. Santo Caracappa santo.caracappa@izssicilia.it<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Vai a Centri di Referenzas<\/a><\/p>\n<\/div>

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