OIE Reference Laboratory for Leishmaniosis. National Reference Centre for Leishmaniosis (C.Re.Na.L.)
Protozoan identification
National Reference Centre for Animal Leishmaniasis performs the serological (IFAT, Elisa) and molecular diagnostic (including Real Time PCR) tests applied to Leishmaniasis such as the isolation, characterisation of strains and production of reference materials. Standard Sera (positive and negative) are available such as DNA standards and Leishmania strains.
Details of available exams are reported in the tables below.
The Standard Operative Procedures (POS/PdP) are certified according to UNI CEI EN ISO/IEC 17025:2005.
Molecular diagnostic
| Pathogen | Matrix | Method | POS/PdP |
| Leishmania Infantum | Needle aspiration lymph node, bone marrow, blood, spleen (fresh, frozen or refrigerated) | Immunofluorescence assay test | POS CRE07 Rev.1 |
| Leishmania Infantum | Needle aspiration lymph node, bone marrow, blood, spleen (fresh, frozen or refrigerated) | Leishmania Isolation | – |
| Leishmania spp. | Extracted DNA | Giemsa staining | – |
| Leishmania spp. | Extracted DNA | Cytotoxis activity evaluation | – |
Serological and antigen identification
| Pathogen | Matrix | Method | POS/PdP |
| Leishmania Infantum | Serum (refrigerated) | Immunofluorescence assay test | PDP CRE02Rev. 5 |
| Leishmania spp. | Needle aspiration lymph node, bone marrow (refrigerated max 72h) | Leishmania Isolation | PDP CRE04
Rev. 5 |
| Leishmania spp. | Smear slide (fresh) | Giemsa staining | – |
| Leishmania spp. | Leishmania protozoan | Cytotoxis activity evaluation | – |
Services
| Immunofluorescence assay (Leishmania Infantum antibody test kit ) |
| Proficiency test organization |
| Technical training |
| Research |
| Browse Catalog | Contact: Fabrizio Vitale fabrizio.vitale@izssicilia.it |